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KMID : 0811720110150030129
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Korean Journal of Physiology & Pharmacology
2011 Volume.15 No. 3 p.129 ~ p.135
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5-Hydroxytryptamine Generates Tonic Inward Currents on Pacemaker Activity of Interstitial Cells of Cajal from Mouse Small Intestine
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Shahi Pawan Kumar
Choi Seok
Zuo Dong-Chuan
Yeum Cheol-Ho
Yoon Pyung-Jin
Lee Jun
Kim Young-Dae
Park Chan-Guk
Kim Man-Yoo
Shin Hye-Rang
Oh Hyun-Jung
Jun Jae-Yeoul
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Abstract
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In this study we determined whether or not 5-hydroxytryptamine (5-HT) has an effect on the pacemaker activities of interstitial cells of Cajal (ICC) from the mouse small intestine. The actions of 5-HT on pacemaker activities were investigated using a whole-cell patch-clamp technique, intracellular Ca2＋ ([Ca2＋]i) analysis, and RT-PCR in ICC. Exogenously-treated 5-HT showed tonic inward currents on pacemaker currents in ICC under the voltage-clamp mode in a dose-dependent manner. Based on RT-PCR results, we found the existence of 5-HT2B, 3, 4, and 7 receptors in ICC. However, SDZ 205557 (a 5-HT4 receptor antagonist), SB 269970 (a 5-HT7 receptor antagonist), 3-tropanylindole ? 3 ? carboxylate methiodide (3-TCM; a 5-HT3 antagonist) blocked the 5-HT-induced action on pacemaker activity, but not SB 204741 (a 5-HT2B receptor antagonist). Based on [Ca2＋]i analysis, we found that 5-HT increased the intensity of [Ca2＋]i. The treatment of PD 98059 or JNK II inhibitor blocked the 5-HT-induced action on pacemaker activity of ICC, but not SB 203580. In summary, these results suggest that 5-HT can modulate pacemaker activity through 5-HT3, 4, and 7 receptors via [Ca2＋]i mobilization and regulation of mitogen-activated protein kinases.
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KEYWORD
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Interstitial cells of Cajal (ICC), Intestinal motility, Pacemaker currents, 5-hydroxytryptamine (5-HT)
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